In popular culture
The film is seen briefly in Women on the Verge of a Nervous Breakdown as the characters portrayed by Carmen Maura and Fernando Guillén are dubbers for the film into Spanish.
Vienna and Jill McBain (Claudia Cardinale) of Sergio Leone's Once Upon a Time in the West have similar backstories (both may be former prostitutes who become saloonkeepers), and both own land where a train station will be built because of access to water. Also, Harmonica (Charles Bronson), like Sterling Hayden's title character, is a mysterious, gunslinging outsider known by his musical nickname. Some of West's central plot (Western settlers vs. the railroad company) may be recycled from Johnny Guitar.
While on the run, the murderous couple in François Truffaut's Mississippi Mermaid (La Sirène du Mississippi), played by Jean-Paul Belmondo and Catherine Deneuve, go to a showing of Johnny Guitar in Lyon and discuss the film in the street afterwards.
In Bonanza, episode 12.1, "The Night Virginia City Died", Vienna's bar is burned down for the umpteenth time. The footage is spectacular, and appears to have been used in many films and TV shows.
In the 1971 western spoof Support Your Local Gunfighter, actor Ben Cooper parodies his own role as a young gunslinger in over his head.
In the Sunn O))) band's album, White1, the song "My Wall" frequently mentions Johnny Guitar in its lyrics.
The movie is mentioned by the main hero of Jean-Luc Godard's 10th feature film, Pierrot Le Fou (1965) and in La Chinoise (1967) by Henri, who gets expelled from the revolutionary cell for defending the movie. In Weekend (1967), 'Johnny Guitar' is a call sign uttered into a walkie-talkie. While in Histoire(s) du cinéma (1988-1998) and The Image Book (2018), some sequences are quoted and overlapped during Godard's examination of cinema history.
Shots from the movie are used in Guy Debord's The Society of the Spectacle.
The title song is heard on radios throughout the Mojave wasteland in the videogame Fallout: New Vegas.
Musician Johnny "Guitar" Watson adopted his stage name after the film.