Film
Harley Quinn was initially set to appear in Batman Unchained, the fifth film planned for the original Batman film series. She was to be featured as the daughter of the Joker (Jack Nicholson) who allies herself with the Scarecrow to get revenge on Batman for her father's death. However, due to the critical and commercial failure of Batman & Robin, this film was cancelled.
The Batman: Arkham version of Harley Quinn makes a cameo appearance in Ready Player One.
Harley Quinn appears in Joker: Folie à Deux, portrayed by Lady Gaga. This version is Harleen "Lee" Quinzel, a psychology student posing as a patient at Arkham State Hospital who becomes obsessed with the Joker and forms a deadly romantic relationship with her idol. She eventually becomes pregnant with Arthur's child and leaves upon learning Arthur has disowned the Joker persona, leaving the future of their child unknown. Describing Quinzel, Todd Phillips noted how this version of the character is manipulative, amoral and "more grounded", with the film ignoring the character's classic mannerisms and style to fit into the world created in Joker (2019).
Dr. Harleen Quinzel / Harley Quinn appears in films set in the DC Extended Universe, portrayed by Margot Robbie.
The character debuted in the 2016 film Suicide Squad. Paul Dini, the creator of Harley Quinn, said Robbie "nailed" her role.
Harley Quinn appears in the 2020 spin-off film Birds of Prey, which Robbie also produced. On Robbie's portrayal of the character in the film, Dini commented: "I think they really got the essence of the character down, and they made her quite a lot of fun and appealing in so many ways. She's not totally the animated version, and it's not totally the Jimmy Palmiotti and Amanda Conner version, but it kind of borrows from all of them and creates its own reality and its own fun. There are so many moments in that movie that I just think are wonderful. [...] When I saw her running, laughing hysterically, pushing a shopping cart full of Peeps, I said, "That's my girl". All those little impish things that she did in the movie – sitting down eating cereal, watching Tweety Bird cartoons, and just kind of skipping through life cheerfully oblivious of the devastation she's caused – that's Harley".
Harley Quinn appears in The Suicide Squad (2021), a standalone sequel to Suicide Squad.
Harley Quinn makes a non-speaking cameo appearance in Justice League: The New Frontier.
An alternate universe version of Harley Quinn appears in Justice League: Crisis on Two Earths. This version is a monkey owned by the Jester.
Harley Quinn appears in Batman: Assault on Arkham, voiced again by Hynden Walch.
A darker version of Harley Quinn, named Harlequin, appears in Justice League: Gods and Monsters, voiced again by Tara Strong. She is a psychotic serial killer who kidnaps, murders, and turns people into human-sized dolls to create a doll family to play with. After already killing a man (father), a woman (mother), a child (little brother), and an old woman (grandma), she attempts to complete her "family" with the murder of a young woman (older sister) but is stopped by the Batman of this universe, Kirk Langstrom, who kills Harlequin by biting her throat and drinking her blood.
Harley Quinn appears in Lego DC Comics Super Heroes: Justice League – Gotham City Breakout, voiced again by Tara Strong.
Harley Quinn makes a non-speaking cameo appearance in Batman: The Killing Joke, via photographs.
Harley Quinn appears in Batman and Harley Quinn, voiced by Melissa Rauch. She works as a waitress after breaking up with the Joker again, and helps Batman and Nightwing stop Poison Ivy from killing all life on Earth. Afterwards, she begins hosting Ask Dr. Quinzel, a reality show where contestants undergo an obstacle course to win a year of therapy.
Dr. Harleen Quinzel appears in Batman vs. Two-Face, voiced by Sirena Irwin. This version is Hugo Strange's assistant, who reciprocates the Joker's flirting. In a Blu-ray exclusive bonus scene, Quinzel, dressed as Harley Quinn, breaks the Joker out of prison.
Harley Quinn appears in DC Super Heroes vs. Eagle Talon, voiced by Kang Ji-young.
Harley Quinn appears in Scooby-Doo! & Batman: The Brave and the Bold, voiced again by Tara Strong.
A feudal Japanese version of Harley Quinn appears in Batman Ninja, voiced by Rie Kugimiya and Tara Strong in Japanese and English, respectively.
Harley Quinn makes a non-speaking cameo appearance in Teen Titans Go! To the Movies.
Harley Quinn appears in Batman vs. Teenage Mutant Ninja Turtles, voiced again by Tara Strong.
Dr. Harleen Quinzel makes a non-speaking cameo appearance in Batman: Death in the Family.
Harley Quinn makes a non-speaking cameo appearance in Space Jam: A New Legacy.
Harley Quinn appears in Injustice, voiced by Gillian Jacobs.
Harley Quinn appears in Teen Titans Go! & DC Super Hero Girls: Mayhem in the Multiverse, voiced again by Tara Strong.
Harley Quinn appears in Scooby-Doo! and Krypto, Too!, voiced again by Tara Strong.
The Batman Ninja version of Harley Quinn appears in Batman Ninja vs. Yakuza League, voiced again by Rie Kugimiya in Japanese and Karlii Hoch in English.
Yo-Yo appears in Justice League: The Flashpoint Paradox, voiced by Hynden Walch.
Harley Quinn appears in Suicide Squad: Hell to Pay, voiced again by Tara Strong. This version is a member of the Suicide Squad whose appearance is inspired by her counterpart from The New 52.
Harley Quinn appears in Batman: Hush and Justice League Dark: Apokolips War, voiced again by Hynden Walch. In the latter film, she becomes the new leader of the Suicide Squad following Amanda Waller's death from cancer and seeks to avenge the Joker after Batman kills him while under Darkseid's control.
Harley Quinn appears in Lego Batman: The Movie – DC Super Heroes Unite, an adaptation of the video game Lego Batman 2: DC Super Heroes, with Laura Bailey reprising her role.
Harley Quinn appears in The Lego Batman Movie, voiced by Jenny Slate.
Harley Quinn appears in The Lego Movie 2: The Second Part, voiced by Margot Rubin.